Design an experiment to confirm that the decolorization of methylene blue in skim milk is catalyzed by a heat-labile enzyme (dehydrogenase).

Here is the hypothesis and procedure for an experiment to confirm the findings of the reductase (dehydrogenase) test: a. Hypothesis: The decolorization of methylene blue in skim milk is catalyzed by a heat-labile enzyme (dehydrogenase), meaning its activity will be significantly reduced or abolished at high temperatures. b. Procedure: Aim: To confirm that the decolorization of methylene blue in skim milk is due to the activity of a heat-labile enzyme (dehydrogenase). Materials: Fresh skim milk (non-sterilized) Methylene blue solution (0.005%) Three clean test tubes Water bath set at 37°C Boiling water bath (100°C) Ice bath (0°C) Stopwatch Measuring cylinder or pipette (for milk) Dropper (for methylene blue) Method: 1. Preparation: Label three clean test tubes as A, B, and C. Add 5 ml of fresh skim milk to each test tube. Add 5 drops of methylene blue solution to each test tube. Gently mix the contents of each test tube. 2. Experimental Setup: Test Tube A (Boiled Control): Place test tube A in the boiling water bath (100°C) for 5 minutes to denature any enzymes present. After boiling, cool the test tube to 37°C (e.g., by placing it in the 37°C water bath). Test Tube B (Optimal Temperature): Place test tube B directly into the water bath set at 37°C. Test Tube C (Low Temperature): Place test tube C into the ice bath (0°C). 3. Observation: Once all test tubes have reached their target temperatures, start the stopwatch simultaneously for all three. Observe each test tube and record the time taken for the methylene blue to completely decolorize (turn colorless). If no decolorization occurs within 60 minutes, record "no decolorization" or "decolorization time > 60 minutes". 4. Data Collection: Record the treatment/temperature and the corresponding decolorization time for each test tube in a table. Expected Results and Confirmation: Test Tube A (Boiled): The methylene blue is expected to remain blue or decolorize very slowly, indicating that the enzyme was denatured by boiling. Test Tube B (37°C): The methylene blue is expected to decolorize relatively quickly, as 37°C is often an optimal temperature for many biological