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Practical work on food test. Activity 1.1.3 from understanding life science. Study the type of reaction on chemical used.
Biology
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Here's a breakdown of the practical food tests, correcting some of the associations in your notes:
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Benedict's Solution
- What it tests for: Reducing sugars (e.g., glucose, fructose, maltose). It does not test for starch or general carbohydrates.
- Procedure: Add Benedict's solution to the food sample and heat gently in a water bath.
- Positive result: The solution changes color from blue to green, yellow, orange, or brick-red, depending on the concentration of reducing sugar.
- Notes' correction: Your notes incorrectly associate Benedict's with starch. Starch is a non-reducing sugar.
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Fehling's Solution (A and B)
- What it tests for: Reducing sugars. Fehling's test is similar to Benedict's test.
- Procedure: Mix equal parts of Fehling's Solution A and B, add to the food sample, and heat gently in a water bath.
- Positive result: The solution changes color from blue to green, yellow, orange, or brick-red precipitate.
- Notes' correction: Your notes incorrectly associate Fehling's with lipids, salts, and oils.
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Iodine Solution
- What it tests for: Starch.
- Procedure: Add a few drops of iodine solution directly to the food sample.
- Positive result: The solution changes color from yellow-brown to blue-black.
- Notes' correction: Your notes incorrectly associate Iodine with proteins.
Here are the correct associations for food tests:
- Benedict's Solution / Fehling's Solution: Tests for reducing sugars.
- Iodine Solution: Tests for starch.
- (Additional common tests not in your notes but relevant):
- Biuret Test: Tests for proteins. Positive result is a color change from blue to violet/purple.
- Emulsion Test: Tests for lipids (fats and oils). Positive result is a cloudy white emulsion forming when the sample is mixed with ethanol and then water.
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