Okay, Delcie, let's complete the remaining parts of this question.
d) Plot a bar chart graph of percentage dilution against time of decolorization:
To plot this graph:
• The x-axis should represent the Percentage Dilution of Stale Milk (e.g., 0%, 20%, 40%, 80%).
• The y-axis should represent the Time of Decolorization (in minutes or seconds).
• Each bar on the graph will correspond to a specific percentage dilution, and its height will indicate the average time taken for methylene blue to decolorize at that dilution.
• Based on the expected results, the bars should generally show an increasing trend in decolorization time as the percentage dilution increases (meaning less concentrated stale milk).
e) Interpret your result as fully as you can:
-
At 0% dilution (100% stale milk concentration):
This sample contains the highest concentration of microorganisms and their reductase enzymes, as well as the highest substrate concentration. Therefore, the decolorization time will be the shortest, indicating the highest rate of enzyme activity.
-
At 20% dilution (80% stale milk concentration):
With a slight dilution, the concentration of enzymes and substrate is slightly reduced compared to 0% dilution. Consequently, the decolorization time will be slightly longer than at 0% dilution, but still relatively fast, as there is still a high level of enzyme activity.
-
At 40% dilution (60% stale milk concentration):
Further dilution leads to a more significant reduction in the concentration of enzymes and substrate. The decolorization time will be noticeably longer than at 20% dilution, as the rate of enzyme activity has decreased due to fewer enzyme-substrate interactions.
-
At 80% dilution (20% stale milk concentration):
At this high level of dilution, the concentration of active enzymes and available substrate is significantly reduced. The decolorization time will be much longer, possibly several times longer than at 0% dilution. This indicates a very low rate of enzyme activity, as there are fewer enzyme molecules to catalyze the reduction of methylene blue, or fewer substrate molecules for the enzymes to act upon.
Last free one today — make it count tomorrow, or type /upgrade for unlimited.