Let's break down this Dihybrid cross.
First, we define the alleles for the two traits:
- For seed shape:
- R = Round (dominant allele)
- r = wrinkled (recessive allele)
- For seed color:
- Y = Yellow (dominant allele)
- y = green (recessive allele)
We are crossing a plant with round and yellow seeds with a plant with wrinkled and green seeds. Assuming these are pure-breeding (homozygous) parents, their genotypes will be:
- Round and Yellow parent: RRYY
- wrinkled and green parent: rryy
Step 1: Parental (P) Generation
- Parent 1 (P1): RRYY (Phenotype: Round, Yellow)
- Parent 2 (P2): rryy (Phenotype: wrinkled, green)
Step 2: Gametes from P Generation
- P1 (RRYY) can only produce one type of gamete: RY
- P2 (rryy) can only produce one type of gamete: ry
Step 3: First Filial (F1) Generation
When the gametes from the P generation combine, all offspring in the F1 generation will have the same genotype and phenotype:
- Cross: RY × ry
- F1 Genotype: RrYy
- F1 Phenotype: All offspring will be Round, Yellow.
Step 4: Gametes from F1 Generation
The F1 individuals (RrYy) are dihybrids. According to Mendel's Law of Independent Assortment, they will produce four types of gametes in equal proportions:
Step 5: Second Filial (F2) Generation
To find the F2 generation, we cross two F1 individuals (or self-pollinate an F1 individual). We use a Punnett square to show the possible combinations of gametes:
| Gametes | RY | Ry | rY | ry |
| :------ | :--- | :--- | :--- | :--- |
| RY | RRYY | RRYy | RrYY | RrYy |
| Ry | RRYy | RRyy | RrYy | Rryy |
| rY | RrYY | RrYy | rrYY | rrYy |
| ry | RrYy | Rryy | rrYy | rryy |
Step 6: F2 Genotypic and Phenotypic Ratios