Purpose of tissue processing is to:

Here are the answers to your questions:

1. Purpose of tissue processing is to: Tissue processing is a series of steps designed to prepare biological tissue for microscopic examination. The primary goal is to replace water in the tissue with a medium, typically paraffin wax, that allows for the cutting of very thin, stable sections (microtomy). The correct option is B.

   • B. Embed tissues in wax for microtomy

2. The first step in tissue processing is: After fixation, which preserves the tissue, the first step in the actual tissue processing sequence is dehydration. This involves removing water from the tissue using ascending concentrations of alcohol. The correct option is B.

   • B. Dehydration

3. Over-processing causes: Over-processing, especially prolonged exposure to dehydrating and clearing agents, can remove too much water and lipids from the tissue, leading to excessive hardening and brittleness. This makes the tissue difficult to section and prone to cracking. The correct option is B.

   • B. Hardening and cracking

4. Automated tissue processors: Automated tissue processors are machines designed to carry out the steps of dehydration, clearing, and infiltration by automatically moving tissue samples through a series of reagents according to a pre-programmed schedule. This improves efficiency, consistency, and reduces manual labor. The correct option is B.

   • B. Move tissues through reagents automatically