A) Propose two possible hypotheses (a general, not specific statements or guess) related to the effect on substrate concentration and enzyme concentration on rate of catalase activity

Here are the answers to Experiment 4:

a) Propose two possible hypotheses (a general, not specific statements or guess) related to the effect on substrate concentration and enzyme concentration on rate of catalase activity

i. Substrate concentration:
If the enzyme (catalase) concentration is kept constant, then increasing the substrate (hydrogen peroxide) concentration will increase the rate of catalase activity (degree of fizzing) until the enzyme becomes saturated.

ii. Enzyme concentration:
If the substrate (hydrogen peroxide) concentration is kept constant, then increasing the enzyme (catalase from potato juice) concentration will increase the rate of catalase activity (degree of fizzing).

b) Record your results in the table below
(These are expected results based on enzyme kinetics principles, as no actual experiment was performed.)

	Test tube A	Test tube B	Test tube C	Test tube D
Potato juice (catalase)	3 ml	2 ml	1 ml	0 ml
Hydrogen peroxide	1 ml	1 ml	2 ml	3 ml
Degree of fizzing	Moderate	Slight	Very slight	None