An experiment was conducted to investigate the effect of substrate concentration on enzyme activity using yeast suspension and stale milk. The procedure involved adding different concentrations of yea

Let's check your answers, Delcie.

a) Suggest a Hypothesis for your experiment:
✅ Correct. Your hypothesis accurately describes the expected relationship between substrate concentration and enzyme activity, including the concept of enzyme saturation.

b) Describe your procedure:
❌ Incorrect. There are two main issues in your procedure:

• You state "add 5ml of yeast suspension to each test tube". To investigate the effect of substrate concentration, you need to add different concentrations (dilutions) of the yeast suspension (or stale milk) to each test tube, not the same amount of a single suspension.
• You state "heat in the water bath for 45 seconds each". The experiment measures the time taken for decolorization, which is a variable. The water bath is used to maintain a constant temperature throughout the observation, not for a fixed heating duration. You should observe and record the time until decolorization occurs for each sample.

c) Observation and results:
✅ Correct. Your observation correctly states that decolorization time depends on the concentration of stale milk and accurately predicts the inverse relationship: higher concentration leads to shorter decolorization time.