I. Adaptation of chloroplast and mitochondria to their functions: Chloroplast: Thylakoids are stacked into grana*, increasing the surface area for light-dependent reactions and maximizing light absorption by chlorophyll. The stroma* contains enzymes necessary for the light-independent reactions (Calvin cycle) to synthesize sugars. Mitochondria: The inner membrane is highly folded into cristae*, which greatly increases the surface area for the electron transport chain and ATP synthase, maximizing ATP production during cellular respiration. The matrix* contains enzymes for the Krebs cycle and link reaction, as well as mitochondrial DNA and ribosomes, facilitating energy production. II. Differentiation between a bacterial and a chlorenchyma cell using electron microscope structures: A chlorenchyma cell (plant cell) possesses a true nucleus enclosed by a nuclear envelope, chloroplasts for photosynthesis, and mitochondria for respiration. It also has a large central vacuole and a cell wall made of cellulose*. A bacterial cell (prokaryote) lacks a true nucleus (having a nucleoid region instead), lacks membrane-bound organelles like chloroplasts and mitochondria, and has a cell wall made of peptidoglycan*. III. Advantages and disadvantages of using light and electron microscopes to study cell organelles: Light Microscope (LM): Advantages: Can view living specimens, relatively inexpensive, and has simple sample preparation*. Disadvantages: Lower resolution and magnification*, limiting the detail of internal organelles. Electron Microscope (EM): Advantages: Offers much higher resolution and magnification*, revealing fine details of internal cell structures and organelles. Disadvantages: Cannot view living specimens (samples must be in a vacuum), is expensive to purchase and maintain, and requires complex sample preparation*.