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Predict the phenotypic and genotypic ratios of the offspring from a cross between a tortoiseshell female cat and an orange male cat.

Biology

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Part 26.1· 3 steps

  1. Determine the genotypes of the parent cats.

    • A female cat with tortoise-shell colour must have both alleles for black and orange fur, so her genotype is XBXO\text{X}^{\text{B}}\text{X}^{\text{O}}.
    • An orange male cat has only one X chromosome, so his genotype is XOY\text{X}^{\text{O}}\text{Y}.
  2. Set up a Punnett square for the cross.

    Parents: XBXO\text{X}^{\text{B}}\text{X}^{\text{O}} (female) ×\times XOY\text{X}^{\text{O}}\text{Y} (male)

    Gametes from female: XB\text{X}^{\text{B}}, XO\text{X}^{\text{O}}
    Gametes from male: XO\text{X}^{\text{O}}, Y\text{Y}

     XOYXBXBXOXBYXOXOXOXOY\begin{array}{|c|c|c|} \hline \text{ } & \text{X}^{\text{O}} & \text{Y} \\ \hline \text{X}^{\text{B}} & \text{X}^{\text{B}}\text{X}^{\text{O}} & \text{X}^{\text{B}}\text{Y} \\ \hline \text{X}^{\text{O}} & \text{X}^{\text{O}}\text{X}^{\text{O}} & \text{X}^{\text{O}}\text{Y} \\ \hline \end{array}
  3. Determine the F1 genotypes and phenotypes and their proportions.

    • Genotypes:

      • 1/41/4 XBXO\text{X}^{\text{B}}\text{X}^{\text{O}}
      • 1/41/4 XBY\text{X}^{\text{B}}\text{Y}
      • 1/41/4 XOXO\text{X}^{\text{O}}\text{X}^{\text{O}}
      • 1/41/4 XOY\text{X}^{\text{O}}\text{Y}
    • Phenotypes:

      • 1/41/4 Tortoise-shell female
      • 1/41/4 Black male
      • 1/41/4 Orange female
      • 1/41/4 Orange male

Part 26.2

Male kittens have only one X chromosome (XY\text{XY}). To express the tortoise-shell colour, an individual needs to have both the XB\text{X}^{\text{B}} (black) and XO\text{X}^{\text{O}} (orange) alleles. Since males only inherit one X chromosome, they can only carry one fur colour allele (XB\text{X}^{\text{B}} or XO\text{X}^{\text{O}}), making it impossible for them to be tortoise-shell.

Part 26.3

Female kittens have two X chromosomes (XX\text{XX}). This allows them to inherit one XB\text{X}^{\text{B}} allele on one X chromosome and one XO\text{X}^{\text{O}} allele on the other X chromosome, resulting in the genotype XBXO\text{X}^{\text{B}}\text{X}^{\text{O}}. The expression of both alleles due to random X-inactivation in different cells leads to the tortoise-shell pattern.

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