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To calculate the enzyme activity, we will follow these steps:

Chemistry

ScanSolve AI Answer

To calculate the enzyme activity, we will follow these steps:

  1. Calculate the rate of change of product concentration using the Beer-Lambert Law.
  2. Calculate the total rate of product formation in the reaction mixture.
  3. Convert the total rate of product formation to International Units (IU).
  4. Calculate the enzyme activity per unit volume of the enzyme solution.

Given values:

  • Rate of change in absorbance, ΔAΔt=0.20 min−1\frac{\Delta A}{\Delta t} = 0.20 \text{ min}^{-1}
  • Molar extinction coefficient, ϵ=6.22×103 M−1cm−1\epsilon = 6.22 \times 10^3 \text{ M}^{-1}\text{cm}^{-1} (assuming standard units for ϵ\epsilon)
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