What is the maximum relative centrifugal force applied when red blood cells are sedimented at 1000 rpm in a rotor of maximum sample radius equal to 10 cm?

The formula for RCF (G-force) is:

$$RCF = 1.12 \times r \times \left(\frac{RPM}{1000}\right)^2$$

Given:
$r = 10 \text{ cm}$
$RPM = 1000 \text{ rpm}$

$$RCF = 1.12 \times 10 \times (1)^2$$ $$RCF = 1.12 \times 10 \times 1$$ $$RCF = 11.2$$

The maximum relative centrifugal force is $\boxed{\text{11.2 G}}$.

• Calculate the G force applied in sedimenting starch suspension at 50 rpm in a rotor of sample radius 200 mm.

The formula for RCF (G-force) is:

$$RCF = 1.12 \times r \times \left(\frac{RPM}{1000}\right)^2$$

Given:
$r = 200 \text{ mm}$
$RPM = 50 \text{ rpm}$

Convert the radius from millimeters to centimeters:

$$r = 200 \text{ mm} = 200 \times \frac{1}{10} \text{ cm} = 20 \text{ cm}$$

$$RCF = 1.12 \times 20 \times (0.05)^2$$ $$RCF = 1.12 \times 20 \times 0.0025$$ $$RCF = 22.4 \times 0.0025$$ $$RCF = 0.056$$

The G force applied is $\boxed{\text{0.056 G}}$.