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List three (3) types of synthetic biodegradable plastics and one (1) microorganism capable of their degradation. List 3 types of natural plastics and one (1) microorganism capable of their degradation.

Biology

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Section A

1a. Distinguish clearly between biodegradation and biodeterioration (5 marks)

Biodegradation is the microbial breakdown of organic compounds into simpler substances like CO2_2, H2_2O, and biomass, which is a natural, beneficial process for recycling nutrients and managing waste.

Biodeterioration is the unwanted microbial degradation of industrial materials (organic or inorganic), causing physical, chemical, or aesthetic damage leading to economic loss.

AspectBiodegradationBiodeterioration
NatureBeneficial, naturalDetrimental, unwanted
TargetOrganic wasteMaterials like food, metals, concrete
OutcomeEnvironmental recyclingEconomic loss, spoilage

1b(i). Classification of biodeterioration (2.5 marks)

Biodeterioration is classified by:

  • Material affected: Food (e.g., mold on bread), textiles (rot), metals (corrosion), wood (decay), concrete (cracking), fuels (sludge).

  • Microbial agents: Bacteria (e.g., sulfate-reducing), fungi (e.g., Aspergillus), actinomycetes, algae, protozoa.

1b(ii). Cost of biodeterioration (2.5 marks)

Costs include:

  • Direct economic loss: $100-200 billion annually worldwide (e.g., food spoilage ~20-30% of production).

  • Maintenance/repair: Corrosion costs ~3-5% of GDP in developed countries (pipelines, ships).

  • Health risks: Mycotoxins in spoiled food.

2. State how 3 microbes colonize concrete (5 marks)

Concrete colonization involves adhesion, biofilm formation, acid production, and penetration.

  1. Thiobacillus thiooxidans (sulfur-oxidizing bacterium)

    Adheres to moist surface →\rightarrow oxidizes S to H2_2SO4_4 →\rightarrow etches CaCO3_3 in cement.

  2. Fusarium oxysporum (fungus)

    Spores germinate on surface →\rightarrow hyphae penetrate pores →\rightarrow secrete organic acids.

  3. Oscillatoria sp.

    (cyanobacterium/alga)
    Photosynthesizes on surface →\rightarrow traps moisture/dust →\rightarrow produces acids, physical expansion.

    Section B (partial from query)

    Mechanisms of microbial corrosion of metals (7.5 marks)

    Microbial corrosion (MIC) involves:

  4. Acid production

    Microbes (e.g., Thiobacillus) produce H+^+ or organic acids, lowering pH:

    \ceM+2H+−>M2++H2\ce{M + 2H^+ -> M^{2+} + H2}
  5. Cathodic depolarization

    Sulfate-reducing bacteria (Desulfovibrio) consume H2_2:

    \ceSO42−+4H2−>S2−+4H2O\ce{SO4^{2-} + 4H2 -> S^{2-} + 4H2O}
  6. Biofilm effects

    Anaerobic zones under biofilm →\rightarrow differential aeration cells.

  7. EPS production

    Exopolysaccharides trap corrosive agents, concentrate ions.

  8. Enzymatic attack

    Proteases/enzymes degrade passive oxide layers.

    6 characteristics of biodeteriogens influencing activity:

    1. Enzyme production: Cellulases, ligninases for substrate attack.

    2. Adhesion capability: Pili, fimbriae, hydrophobins for surface attachment.

    3. Metabolic versatility: Aerobic/anaerobic, use diverse C/N sources.

    4. Acid/alkali tolerance: Thrive at pH 2-10.

    5. Biofilm formation: Protects from biocides, concentrates nutrients.

    6. Spore-forming: Resistant dormant stage for colonization.

    Section C

    5a. List 3 types of synthetic biodegradable plastics and 1 microorganism capable (4 marks)

    1. Polylactic acid (PLA): From corn starch.

    2. Polyhydroxyalkanoates (PHA): Bacterial polyester.

    3. Polycaprolactone (PCL): Semi-crystalline polyester.

    Microorganism: Amycolatopsis orientalis degrades PLA via hydrolases.

    5b. Natural plastics and 1 microorganism (4.5 marks)

    Natural plastics: Cellulose, chitin, natural rubber (polyisoprene).

    Microorganism: Streptomyces coelicolor degrades natural rubber via latex-clearing protein (Lcp).

    Final answers bolded above.

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