Prepare Nitution of 1:20 Sample for TLC. and the Procedures. 2) prepare Turks Diluting Fluid.

Here are the answers to your questions: 1) Prepare dilution of 1:20 sample for TLC and the procedures. a) Preparation of 1:20 dilution for TLC: The standard dilution for Total Leukocyte Count (TLC) using a WBC pipette is approximately 1:20 (precisely 1:22). Step 1: Draw well-mixed anticoagulated blood (or capillary blood) up to the 0.5 mark in a WBC pipette. Step 2: Wipe any excess blood from the outside of the pipette tip. Step 3: Draw Turk's diluting fluid up to the 11 mark in the same pipette. This creates a dilution of 1 part blood to 21 parts diluting fluid, resulting in a total dilution of (11)/(0.5) = 22, commonly referred to as 1:20 or 1:22. Step 4: Mix the contents thoroughly by rotating the pipette between your palms for 2-3 minutes. b) Procedures for Total Leukocyte Count (TLC): • Sample Collection: Obtain a fresh capillary blood sample or use anticoagulated venous blood. • Dilution: Perform the 1:20 (or 1:22) dilution as described above using a WBC pipette and Turk's diluting fluid. • Mixing: Mix the diluted blood thoroughly in the pipette for 2-3 minutes. • Discarding First Drops: Discard the first 2-3 drops from the pipette to ensure only well-mixed fluid is used. • Charging the Hemocytometer: Carefully charge both sides of a clean Neubauer counting chamber by touching the tip of the pipette to the edge of the coverslip and allowing the fluid to flow in by capillary action. • Settling: Place the charged hemocytometer in a moist chamber for 5-10 minutes to allow the cells to settle. • Counting: Place the hemocytometer on a microscope stage. Using the 10× objective, count the leukocytes in the four large corner squares (W squares) of the counting chamber. • Calculation: Calculate the total leukocyte count using the formula: WBC count = Number of cells counted × Dilution factorArea counted × Depth For the four large corner squares, the area is 4 mm^2 and the depth is 0.1 mm. The dilution factor is 22. WBC count = N × 224 mm^2 × 0.1 mm = N × 220.4 = N × 55 Where N is the total number of WBCs counted in the four large squares. 2) Prepare Turk's Diluting Fluid Turk's diluting fluid is used to lyse red blood cells and stain the nuclei of white blood cells, making them easier to count. Step 1: Measure 1 mL of glacial acetic acid. This component lyses red blood cells. Step 2: Measure 1 mL of 1% aqueous gentian violet solution. This component stains the nuclei of white blood cells, making them visible. Step 3: Add 98 mL of distilled water. This acts as the diluent. Step 4: Mix all components thoroughly and store in a clean, stoppered bottle. The final answer for the preparation of Turk's Diluting Fluid is: Glacial acetic acid: 1 mL 1% aqueous gentian violet: 1 mL Distilled water: 98 mL Send me the next one 📸