We read your photo as:
Describe the procedure for carrying out packed cell volume experiment.
Biology
ScanSolve AI Answer
Step-by-step solution
4 stepsDescribe the procedure for carrying out packed cell volume experiment.
- Collect blood into a heparinized capillary tube, filling it to about two-thirds capacity.
- Seal one end of the capillary tube with clay or by heating it in a flame.
- Place the sealed tube into a microhematocrit centrifuge with the sealed end facing outwards.
- Centrifuge the tubes at 10,000-12,000 rpm for 5 minutes.
- Remove the tube and place it on a microhematocrit reader. Read the percentage of the packed red cell column relative to the total blood column.
Describe the procedures for collection of capillary blood.
- Select a suitable site, typically the fingertip (ring or middle finger) for adults, or the heel for infants.
- Cleanse the chosen site thoroughly with an alcohol swab and allow it to air dry completely.
- Puncture the skin with a sterile lancet, making a quick, firm stab.
- Wipe away the first drop of blood with a clean gauze pad, as it may contain tissue fluid.
- Collect subsequent drops of blood into a capillary tube or onto a slide, avoiding excessive squeezing of the finger.
- Apply gentle pressure with a clean gauze pad to the puncture site until bleeding stops.
Highlight the difference between the Ivy's and Duke's methods of bleeding time.
- Ivy's Method: This method involves making a standardized incision (e.g., 1 mm deep, 10 mm long) on the forearm using a specialized device, while a blood pressure cuff is inflated to 40 mmHg on the upper arm to standardize venous pressure. It is considered more sensitive and reproducible.
- Duke's Method: This method involves making a puncture on the earlobe or fingertip with a lancet. The depth of the puncture is less standardized, and no blood pressure cuff is used. It is less sensitive and less reproducible compared to the Ivy's method.
What are the factors that affects bleeding time?
- Platelet Count: A low platelet count (thrombocytopenia) significantly prolongs bleeding time.
- Platelet Function: Impaired platelet function, due to inherited disorders (e.g., Glanzmann's thrombasthenia, Bernard-Soulier syndrome) or medications (e.g., aspirin, NSAIDs), prolongs bleeding time.
- Vascular Integrity: Defects in the blood vessel walls or surrounding connective tissue can lead to prolonged bleeding time.
- Von Willebrand Factor: Deficiency or dysfunction of von Willebrand factor, which is crucial for platelet adhesion to the injured vessel wall, prolongs bleeding time.
- Temperature: Extreme temperatures, particularly hypothermia, can impair platelet function and prolong bleeding time.
Was this helpful?
Still stuck on this one?
Ask a follow-up, and the answer starts from this question.
Got a different question?Ask your own question
More Biology Questions
QWrite the methodology of RBC count testQChapter 6: Blood cell count, chapter 7: Differential count, chapter 8: Reticulocyte count, chapter…QCalculate the Total WBC count given 160 cells in 4 corner squares and a dilution factor of 20. Also…QWhich solution is use for the counting of WBCsQDetermine the average volume of solution P used.