What are the limitations of using an electron microscope?

Here is the answer to question 2(a):

2. a)

Step 1: State the limitation of the electron microscope.

• Electron microscopes require samples to be in a vacuum, meaning living specimens cannot be observed.
• The preparation process for samples often involves fixation, dehydration, and coating with heavy metals, which can introduce artifacts and alter the natural state of the specimen.
• Electron microscopes produce monochromatic (black and white) images.