State how each of the following parts of the light microscope contributed to clarity of the image of the section observed: (i) Mirror (ii) Diaphragm (iii) Condenser

1. (c) State how each of the following parts of the light microscope contributed to clarity of the image of the section observed:

• (i) Mirror: The mirror reflects light from the light source upwards through the aperture and onto the specimen, providing essential illumination for image formation and clarity.
• (ii) Diaphragm: The diaphragm regulates the amount of light passing through the specimen, which helps to control the brightness and contrast of the image, thus enhancing its clarity.
• (iii) Condenser: The condenser focuses and concentrates the light rays onto the specimen, ensuring uniform and intense illumination. This improves the resolution and overall clarity of the observed image.

2. Using the provided materials, investigate the enzyme-oxygen reaction using the procedure below:

The provided steps I, II, and III describe the preparation of the banana portions. To investigate the enzyme-oxygen reaction using the given materials, the procedure would continue as follows:

• Step 1: Place banana portion 'a' into Beaker G, which contains 50 ml of dilute hydrochloric acid.
• Step 2: Place banana portion 'b' into Beaker H, which contains 50 ml of distilled water.
• Step 3: Place banana portion 'c' into Beaker J (empty), ensuring it is exposed to the air.
• Step 4: Immediately start the stopwatch to begin timing the experiment.
• Step 5: Observe and record the time taken for browning to appear, or the degree of browning, in each banana portion at regular intervals (e.g., every 5 minutes) for a predetermined period.