Describe an experiment to show how reducing sugar can be tested in a germinating bean seed

Question

ScanSolve AI Tutor · Accepted Answer

To test for reducing sugar in a germinating bean seed, you would use the Benedict's test, which detects the presence of reducing sugars by changing color when heated. Here's how to conduct the experiment: Step 1: Prepare the bean seed extract. Take several germinating bean seeds and grind them thoroughly using a mortar and pestle. Add a small amount of distilled water to the ground seeds to create a suspension. Filter the suspension using a funnel and filter paper to obtain a clear bean extract. This extract contains the soluble substances from the seeds. Step 2: Set up the test. Pour about 2 cm³ of the bean extract into a clean test tube. Add an equal volume (about 2 cm³) of Benedict's solution to the test tube. For comparison, set up a control test tube with 2 cm³ of distilled water and 2 cm³ of Benedict's solution. Step 3: Heat the mixture. Place both test tubes into a hot water bath (around 80-100°C) for 5-10 minutes. Step 4: Observe and interpret results. Observe any color changes in both test tubes. If reducing sugars are present in the bean extract, the Benedict's solution will change color from blue to green, then yellow, orange, and finally a brick-red precipitate. The intensity of the color indicates the amount of reducing sugar. The control test tube (water + Benedict's) should remain blue, confirming that the color change is due to the bean extract.

Describe an experiment to show how reducing sugar can be tested in a germinating bean seed

Answer

Step-by-step solution

Prepare the bean seed extract*.

Set up the test*.

Heat the mixture*.

Observe and interpret results*.

Still stuck on this one?

More Biology Questions