Which solution is use for the counting of WBCs

The most common diluent for WBC counting is acetic acid solution, typically a 1-3% concentration.

Here's why:

1. RBC Lysis: Acetic acid dissolves the hemoglobin in RBCs, causing them to burst. This clears the field of view, making it easier to see and count the larger WBCs.
2. WBC Preservation: WBCs are more resistant to the acidic environment and remain intact, allowing for accurate counting.

Sometimes, a specialized WBC diluent containing gentian violet or other stains might be used to make the WBC nuclei more visible.

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