Line Weaver burk equation of enzyme kinetics

The equation is:

$$\frac{1}{v_0} = \frac{K_m}{V_{max}} \cdot \frac{1}{[S]} + \frac{1}{V_{max}}$$

Where:

• $v_0$ is the initial reaction velocity.
• $K_m$ is the Michaelis constant.
• $V_{max}$ is the maximum reaction velocity.
• $[S]$ is the substrate concentration.

When $1/v_0$ is plotted against $1/[S]$ (a Lineweaver-Burk plot or double reciprocal plot), it yields a straight line.

• The y-intercept is $1/V_{max}$.
• The x-intercept is $-1/K_m$.
• The slope of the line is $K_m/V_{max}$.